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The Inoue Method for Preparation and Transformation of

How would treatment with iPS cells work? This makes them rewind to an embryonic state. Then, we can make iPS cells differentiate into the cell type we need by injecting proteins that instruct embryonic stem cells to become liver, retina or any other type of cell. The idea is that these reprogrammed cells can then be inserted in the body to replace damaged cells. We are at least 20 years from any clinical treatments, but the potential is exciting. Are there any potential pitfalls with iPS cell treatments?

Organelles in eukaryotic cells

Yes, we have to be very careful because iPS cells multiply endlessly. This means that if any undifferentiated iPS cells were accidentally put into someone, they could cause tumours. It is not a clinical trial, but a six-subject pilot study to confirm the safety of putting cells derived from iPS cells into humans. Who are the participants in the study?

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The six people all have age-related macular degeneration in their eyes. This weakens the vision in the central field, eventually leaving people with only peripheral vision. In the type of degeneration we are working with, this is caused by the deterioration of the retinal pigment epithelium RPE — the layer of cells that clears away extra-cellular debris that lands on the retina.

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It will take a year to grow enough RPE cells to introduce them to a damaged eye. Although I am excited to see if there is any improvement in sight, this study aims only to demonstrate the safety of RPE cells derived from IPS cells. How confident are you that the pilot will be a success? Very confident.

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We have trialled this intervention on mice, rats and monkeys, and observed no tumours. I chose to work with RPE cells because of their characteristic brown pigment.

This means we can avoid injecting tumour-causing iPS cells by selecting only the clumps of pure brown RPE cells. Of course, we do have to pick out around 50, RPE cells, so it can be a bit tough.

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I’m Pro-Life and Oppose Embryonic Stem Cell Research

Please review our Terms and Conditions of Use and check box below to share full-text version of article. Abstract Optogenetics is a powerful method for studying dynamic processes in living cells and has advanced cell biology research over the recent past. Citing Literature. Related Information. Close Figure Viewer. Browse All Figures Return to Figure. Previous Figure Next Figure.


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